Figure 2.

Adult isolation behavior battery. (A) Experimental timeline. (B) Females spend more time exploring the open arms of the EPM, but adult SI does not influence this measure (left); there is no difference in general locomotor behavior, measured by the number of entries into the closed arms (right). (C) There are no effects of sex or adult SI on the percent time spent exploring the center of the open field (OF; left), however, there is a sex-dependent effect of adult SI on the total distance traveled in the OF (right). (D) Adult SI does not alter preference for a novel social partner over an empty cup in the social interaction test (left) but does decrease total time spent interacting with the social partner and empty cup, an effect driven by males (right). GH males also spend more total time exploring both objects compared to GH females. (E) Adult SI decreases the percent time spent exploring the light side of the light/dark box in both males and females. (F) In the novel object interaction test, all groups except GH males display a preference for a novel vs. familiar object (left), however, this is driven by greater overall interaction time with both objects in males (right). (G) Adult SI mice show delayed fear acquisition compared to GH mice. Data are expressed as means + SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001 between groups; ^#p < 0.05 compared to null hypothesis of preference score = 1.