Table 4A.
Inhibitory effect of TRYP and TRYP-Ox on IL-1β-induced IL-6 and MMP-1/3 secretion by monocytic THP-1 cells, synovial SW982 cells, and HUVEC.
| Compound | THP-1 | SW982 | HUVEC | FLS | SW-982 | HUVEC | FLS | SW982 | HUVEC | FLS |
|---|---|---|---|---|---|---|---|---|---|---|
| IL-6 | MMP-1 | MMP-3 | ||||||||
| IC50 (µM) | ||||||||||
| TRYP | 9.3 ± 2.7 | 2.5 ± 1.1 | 6.4 ± 2.7 | 14.8 ± 1.8 | 7.8 ± 2.0 | 3.4 ± 1.1 | 1.2 ± 0.4 | 8.7 ± 0.3 | 12.8 ± 4.4 | 12.7 ± 4.6 |
| TRYP-Ox | 0.3 ± 0.12# | 0.4 ± 0.2# | 0.9 ± 0.3# | 7.4 ± 1.2# | 2.6 ± 0.8# | 2.9 ± 0.1 | 0.7 ± 0.1 | 1.4 ± 0.1# | 15.9 ± 5.8 | 3.8 ± 0.4# |
The cells were treated with compounds for 30 min and then activated with 5 ng/ml IL-1β. After 24 h incubation, MMP-1/3 and IL-6 were evaluated in the supernatants by ELISA. IC50 values are presented as the mean ± S.D. of three to four independent experiments. #p < 0.05 compared with TRYP.