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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: Biochemistry. 2020 May 4;59(19):1793–1799. doi: 10.1021/acs.biochem.0c00292

Figure 1.

Figure 1.

Lyso-PS and PS lipid profiles of LPCAT3-deficient THP-1 cells treated with the ABHD12 inhibitor DO264. A) Lyso-PS acyltransferase activity of membrane lysates (0.05 mg/mL) from LPCAT3-wild type (sgCtrl) and LPCAT3-null (sgLPCAT3–1-3) THP-1 cells generated by the CRISPR/Cas9 system were tested using 17:1 lyso-PS and C20:4 CoA as substrates. Data represent mean ± SE values. n = 3 per group. B, C) Lyso-PS (B) and PS (C) lipid profiles of LPCAT3-wild type and LPCAT3-null THP-1 cells treated with DMOSO or DO264 (1 μM, 4 h). C20:4 PS lipids are highlighted in red. Data represent mean ± SE values. n = 3 per group. *P < 0.05; ** or ## P < 0.01; *** or ### P < 0.001. (asterisk (*) used for two-sided Student’s t-test performed relative to sgCtrl for (A) and to respective DMSO controls for (B) and (C). Pound (#) used for two-sided Student’s t-test performed for DO264-treated sgLPCAT3 cell populations relative to DO264-treated sgCtrl cells.