Table 1. Summary of the LC-MS/MS assay validation data provided by kit manufacturer.
|
Analyte, unit (polarity: MRM1; MRM2) |
Reagent/ Manufacturer |
Assay range |
Limit of quantification |
Inter-assay precision, CV% |
Recovery, % |
|---|---|---|---|---|---|
| Aldosterone, nmol/L (-:359®331;359®189) |
MassChrom Steroids in Serum and Plasma/ Chromsystems |
0.039–16.62 | 0.039 | 5.9% | 95% |
| Cortisol, µmol/L (+:363®97;363®121) |
0.004–1.44 | 0.004 | 5.0% | 93% | |
| DHEAS, µmol/L (-:369®99;369®82) |
0.066–48.78 | 0.066 | 6.9% | 90% | |
| Testosterone, nmol/L (+:289®97;289®109) |
0.02–83.28 | 0.02 | 8.9% | 95% | |
| Progesterone, nmol/L (+:315®97;315®109) |
0.095–79.5 | 0.095 | 10.2% | 82% | |
| OHP, nmol/L (+:331®109;331®97) |
0.12–90.9 | 0.12 | 9.5% | 92% | |
| Both MRM channels were validated using two different LC-MS/MS instruments. LC-MS/MS - liquid chromatography coupled to the tandem mass spectrometry. MRM - multiple reaction monitoring. DHEAS - dehydroepiandrosterone sulfate. OHP - 17-hydroxyprogesterone. | |||||