Table 1. Comparison of modern techniques used for detection of cancer mutations.

	Sanger sequencing	NGS	qPCR	FISH	CISH
Tumour biopsy	gDNA	gDNA	gDNA	gDNA in fixated cells	gDNA in fixated cells
Liquid biopsy	cfDNA	cfDNA	cfDNA	/	/
Sequence information	partial sequence	sequence	partial sequence	point mutation	point mutation
Time of analysis	7 days	3 days	4h	4h	4h
Precision	nucleotide resolution	nucleotide resolution	mutation resolution	mutation resolution	mutation resolution
Possibility of simultaneous sample analysis	-	+	+	-	-
Possibility of simultaneous gene analysis	+	+	-	+ (a few)	-
Costs of a few sample analysis	high	high	low	medium	low
Costs of high throughput analysis	high	low	low	medium	medium
+ – possible. - – not possible. NGS – next generation sequencing. qPCR – quantitative polymerase chain reaction. FISH – fluorescence in situ hybridization. CISH – chromogenic in situ hybridization. gDNA – genomic DNA. cfDNA – circulating cell-free DNA.