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. Author manuscript; available in PMC: 2020 Jul 31.
Published in final edited form as: Mol Microbiol. 2011 Nov 20;82(6):1545–1562. doi: 10.1111/j.1365-2958.2011.07907.x

Fig. 6.

Fig. 6.

sRNA competition for negative regulation of eptB. Cells harbouring a PBAD–eptB–lacZ translational fusion, which is negatively regulated by MgrR, were used for examining Hfq competition on a negatively regulated target. The cells were grown in LB containing ampicillin, 0.02% arabinose and 100 μM IPTG. The results were plotted as described in Fig. 4. The basal activity was that seen with the plac vector control plasmid for a given strain. Strains used were (A) WT (KM125); basal activity was 3.3 units; (B) ΔmgrR mutant (KM225); basal activity was 2.7 units.