Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 30;8(2):e000691. doi: 10.1136/jitc-2020-000691

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Generation and binding of human CLEC9A-NY-ESO-1, DEC-205-NY-ESO-1 and control-NY-ESO-1 chimeric Ab. (A) Diagram of chimeric Ab consisting of rat or mouse variable regions specific for human CLEC9A, DEC-205, or β-galactosidase (control), and human IgG4 κ constant regions genetically fused to an antigenic sequence of NY-ESO-1. (B) Binding of CLEC9A-NY-ESO-1 (blue, left panel), DEC-205 (red, right panel) to 293 F cells transfected with CLEC9A (left) or DEC-205 (right) and untransfected controls (gray). (C) Binding of chimeric Abs to human Ag presenting cells, shown as representative histograms from one donor and the median fluorescence intensity±SD compiled from five individual healthy donors. Ab, antibody; Ag, antigen; MFI, median fluorescence intensity; NY-ESO-1, New York esophageal squamous cell carcinoma 1.