FIG 5.

(A) Type I hsdS gene showing the location of the 5′- and 3′-TRDs and the inverted repeats. Sequence analysis of representative examples of each hsdS gene present in Enterococcus faecalis. Alignments were carried out using ClustalW and visualized in JalView overview feature. Blue color indicates nucleotide identity, with each column representing one nucleotide. (B) The whole-genome phylogenic tree was constructed by CVTree (version 3.0.0) for 34 strains of Enterococcus faecalis annotated in REBASE. Red circles indicate strains with Type I systems that include duplicated hsdS genes containing inverted repeats. The horizontal distance reflects the dissimilarity of each strain.