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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Kidney Int. 2019 Nov 26;97(4):753–764. doi: 10.1016/j.kint.2019.11.011

Figure 2: Zhx2 deficient mice and Adriamycin Nephrosis.

Figure 2:

(a) Results of real time PCR studies showing significantly lower Zhx2 mRNA expression in BALB/cJ mouse glomeruli compared to BALB/c and C57BL/6 glomeruli. (b) Confocal images of BALB/c and BALB/cJ mouse glomeruli showing reduced Zhx2, and increased ZHX1 and ZHX3, expression in BALB/cJ mice. (c) Morphometric quantification of ZHX2 expression in confocal images in panel b. (d) Morphometric quantification of ZHX1 expression in confocal images in panel b. (e) Morphometric quantification of ZHX3 expression in confocal images in panel b. (f) Partial depletion of glomerular ZHX2 in Zhx2 flox flox, NPHS2 cre+/+ mice by confocal imaging. (g) Albuminuria after induction of low dose Adriamycin nephrosis in BALB/c and BALB/cJ mice (n = 8 mice per group). (h) Podocyte nuclear expression of ZHX3 (arrows in left and amplified middle panels) and ZHX1 (right panels) on Day 14 of adriamycin nephrosis in BALB/c and BALB/cJ mice. (i) Morphometric quantification of increased podocyte nuclear ZHX3 expression as ZHX3:WT1 ratio in BALB/cJ mice represented in panel e. * P<0.05; ** P < 0.01; *** P<0.001. Scale bars: 9.1 μm (b), 10.2 μm (f), 12.5 μm (h left and right panels), 7.9 μm (h middle panel).