Skip to main content
. 2020 Jul 31;15(7):e0236348. doi: 10.1371/journal.pone.0236348

Fig 3. RT-qPCR quantification of relative expression levels of selected differentially expressed genes in dehydrated vocal folds.

Fig 3

The differential expression of 10 genes in dehydrated vocal folds identified by RNA-Seq was validated by RT-qPCR. (A) Cell junction-related genes: desmoglein 1 (DSG1), cadherin 3 (CDH3), nectin cell adhesion molecule 1 (NECTIN1), and syndecan 1 (SDC1). (B) Epidermal differentiation-related genes: S100 calcium-binding protein A9 (S100A9) and serine peptidase inhibitor, Kazal type 5 (SPINK5). (C) Extracellular matrix protein 1 (ECM1) gene. (D) Pro-inflammatory cytokine genes: interleukin 1 alpha (IL1A) and 36 alpha (IL36A). (E) Transcription factor nuclear receptor subfamily 4 group A member 3 (NR4A3) gene. The gene expression levels of the control group were set to 1, and relative expression levels were calculated relative to the HPRT1 gene using the method 2-ΔΔCt. Bars show mean ± SEM. *p-value ≤ 0.05. The detailed results of the qPCR analysis are listed in Table 2.