Table 1. Quantitative PCR primers for RNA-Seq validation.
| Gene symbol | Gene name | Forward (5' - 3') | Reverse (5' - 3') | Amplicon length (base pairs) |
|---|---|---|---|---|
| HPRT1* | hypoxanthine phosphoribosyl-transferase 1 | GATGGTCAAGGTCGCAAGCC | TCCAACAAAGTCTGGCCTGT | 73 |
| DSG1 | desmoglein 1 | TCCTGCTGGCATCGGATTAC | ATAGTGGCCAAACCAGTGGG | 195 |
| CDH3 | cadherin 3 | TGACAACCAAGAGGGGCTTG | ATCCTCTACGTGGACCACCA | 131 |
| CLDN7 | claudin 7 | TACGACTCTGTGCTCGCCCT | CAGCAAGACCTGCCACGATGAA | 199 |
| NECTIN1 (PVRL1) | nectin cell adhesion molecule 1 | AGTACCACTGGACCACGCTG | AGGAGACGGGGTGTAGGGAA | 191 |
| CAMSAP3 | calmodulin regulated spectrin associated protein family member 3 | GCCCGAGTACACAGGTCCTC | CGTGTACAGGGCTCGGAACT | 211 |
| PLEKHA7 | pleckstrin homology domain containing A7 | CAATGAGGAGGCGGCTACGA | GGCTCCACCCACCAGAGTTT | 111 |
| ECM1 | extracellular matrix protein 1 | GGCAGCCATCCCCGAACAA | GGGAGCTGGCTCTTCTTCTGT | 214 |
| SDC1 | syndecan 1 | GGGAGCCGGACTTCACTTTC | GCTGCCTTCGTCCTTCTTCT | 236 |
| GJB2 | Gap junction protein beta 2 | TTGGGGTGCGTGAGTGATGT | CTGCGCTTGCCACCAGTAAC | 78 |
| S100A9 | S100 calcium binding protein A9 | CCTCAAGAAGGAGGCGAGGG | AGCTGCTTGTCCTGGTTCGT | 78 |
| SPINK5 | serine peptidase inhibitor, Kazal type 5 | TGTGGAGATGATGGCCAGACG | ATTTGTCAGATGCAGGCAGGC | 154 |
| NR4A3 (NOR1) | nuclear receptor subfamily 4 group A member 3 | TTCTGACGGCCTCCATTGAC | AGCAGTGTTCGACCTGATGG | 149 |
| IL1A | interleukin 1 alpha | ATCTGGGCGATGCAGTGAAA | CCTGGGTGTCTCAGGCATTT | 158 |
| IL36A | interleukin 36 alpha | CAGGTGTGGGTCGTTCAGGA | GCTAACAGTGGCTGGAACCAT | 75 |
*HPRT1 was used as the endogenous control to calculate the relative expression levels of each tested gene.