Table 2. Summary of qPCR results of selected differentially expressed genes in vocal folds of systemic dehydrated rabbits compared to controls.
| Gene symbol | RNA-Seq fold change | qPCR fold change | qPCR p-value* |
|---|---|---|---|
| DSG1 | -1.99 | -2.37 | 0.0485 |
| CDH3 | -2.32 | -2.86 | 0.0224 |
| CLDN7 | -1.95 | -1.60 | 0.0704; ns |
| NECTIN1 | -2.23 | -1.89 | 0.0394 |
| CAMSAP3 | -1.97 | -1.66 | 0.0754; ns |
| PLEKHA7 | -1.99 | -2.23 | 0.0541; ns |
| ECM1 | -2.16 | -2.35 | 0.0191 |
| SDC1 | -2.19 | -1.97 | 0.0374 |
| GJB2 | -2.83 | -1.80 | 0.0854; ns |
| S100A9 | -6.63 | -4.14 | 0.0398 |
| SPINK5 | -2.75 | -2.39 | 0.0357 |
| NR4A3 (NOR1) | +23.76 | +7.92 | 0.0136 |
| IL1A | -3.65 | -12.72 | 0.0368 |
| IL36A | -2.92 | -2.37 | 0.0381 |
Negative and positive values of fold change indicate downregulation and upregulation of gene expression, respectively.
*Exact p-value of One-tailed Welch′s t-test. ns: non-significant p-value.