. Author manuscript; available in PMC: 2021 Feb 13.

Published in final edited form as: J Med Chem. 2020 Jan 16;63(3):1032–1050. doi: 10.1021/acs.jmedchem.9b01218

Table 5.

Mouse liver microsomal (MLM) stability of selected novel EP2 antagonists^a

Compd.	MLM T1/2 (minutes)
3a	<2
8c	2.9
8d	13
8g	2.9
14a	6.5
20b	8
20c	17
20e	12
20f	96
20h	16
20o	17
24a	17
24c	26
24e	15
24i	56

The compounds at 1μM were incubated in 0.125 mg/mL liver microsomes that are activated with addition of NADPH (final concentration 1 mM) and quenched the reaction at 0, 5, 15, 30, 60 and 120 min using ice-cold acetonitrile. The % compound remaining was measured using LC-MS/MS from which half-life was calculated. The work was done at CRO laboratories.