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. 2020 Jul 31;6(31):eaba0588. doi: 10.1126/sciadv.aba0588

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 1 — (A) Reaction scheme for conjugation of fluorescent dye and peptides on HA backbone using EDC/sulfo-NHS chemistry. (B) ¹H nuclear magnetic resonance (NMR) spectrum shows the presence of characteristic peaks b, c, d, and e from the peptides, indicating their successful conjugation to HA. (C) GPC profile of HAPPI shows the appearance of ultraviolet (UV) absorbance at the elution time of conjugates [refractive index (RI) signals], in contrast to the profile of fluorescent HA alone, further validating the chemical conjugation. (D) Image of freeze-dried HAPPI as the bluish material in the vial. Topographic air tapping mode atomic force micrographs for HA (E), HA-VBP (F), HA-CBP (G), and HAPPI (H) (scale bars, 500 nm). Photo credit: (D) Y. Gao, Harvard University.