Skip to main content
. 2020 Jul 13;9:e58943. doi: 10.7554/eLife.58943

Figure 3. Endogenously-tagged γ-tubulin-GFP localises to the somatic Golgi of sensory neurons.

(A) Confocal images show the somas and some proximal dendrites of sensory neurons within the dorsal cluster: class I (yellow), class IV (blue), as (pink) and other (orange) from a 3rd instar larva expressing endogenously-tagged γ-tubulin-GFP and immunostained for GFP (green) and HRP (marking Golgi stacks, greyscale). Upper panel (A) shows an overlay of the GFP and HRP signals, lower panel (A’) shows only the GFP signal with coloured neuronal outlines drawn for clarity. (B, C) Confocal images show the somas of class I (B) or class IV (C) da neurons from a 3rd instar larva expressing endogenously-tagged γ-tubulin-GFP fixed and immunostained for GFP (green), GM130 (magenta) and HRP (greyscale). Enlarged boxes in (B) and (C) show side-on and face-on stacks, respectively. (D) Confocal images show an example of a single Golgi stack within a da neuron from a 3rd instar larva expressing endogenously-tagged γ-tubulin-GFP fixed and immunostained for GFP (green), Arl1 (trans-Golgi, magenta) and GM130 (cis-Golgi, greyscale). The larva was also stained with HRP antibodies to identify neurons, but this channel has been omitted for clarity.

Figure 3.

Figure 3—figure supplement 1. Endogenously-tagged γ-tubulin-GFP localises to the somatic Golgi of sensory neurons.

Figure 3—figure supplement 1.

(A,B) Confocal images show the soma of an es neuron (A) or another sensory neuron within the dorsal cluster (B) from 3rd instar larva expressing two copies of endogenous γ-tubulin-GFP fixed and immunostained with antibodies against GFP (green), GM130 (magenta) and HRP (greyscale). γ-tubulin-GFP and GM130 signals associate at presumptive side-on stacks (extended GM130 signal) and face-on stacks (round GM130 signal) in a way that suggests γ-TuRCs are recruited to the rims of the cis-Golgi.