Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w1118 | Bloomington Drosophila Stock Center | BDSC:3605 | |
| Genetic reagent (D. melanogaster) | rim103 | PMID:23175813 | ||
| Genetic reagent (D. melanogaster) | kis1 | Bloomington Drosophila Stock Center | BDSC:431 | |
| Genetic reagent (D. melanogaster) | chd1[1] and chd1 [1], chd1[wt] |
PMID:21177652 | ||
| Genetic reagent (D. melanogaster) | ash1-mimic | Bloomington Drosophila Stock Center | BDSC:23524 | |
| Genetic reagent (D. melanogaster) | bchs58 | Bloomington Drosophila Stock Center | BDSC:9887 | |
| Genetic reagent (D. melanogaster) | UAS-CREG | this paper | Fly carrying UAS-Creg transgene | |
| Genetic reagent (D. melanogaster) | wrd104 | PMID:16957085 | ||
| Genetic reagent (D. melanogaster) | pdk1 | PMID:21930778 | ||
| Genetic reagent (D. melanogaster) | ok371-gal4 | PMID:16378756 | ||
| Genetic reagent (D. melanogaster) | tubulin-gal4 | PMID:21930778 | ||
| Genetic reagent (D. melanogaster) | 3rd chromosome deficiency collection | Bloomington Drosophila Stock Center | ||
| Genetic reagent (D. melanogaster) | Creg-m1 | Bloomington Drosophila Stock Center | BDSC:42140 | |
| Genetic reagent (D. melanogaster) | Creg-m2 | Bloomington Drosophila Stock Center | BDSC:22800 | |
| Genetic reagent (D. melanogaster) | MN1b-gal4 | Bloomington Drosophila Stock Center | BDSC:40701 | |
| Genetic reagent (D. melanogaster) | MN1s-gal4 | Bloomington Drosophila Stock Center | BDSC:49227 | |
| Genetic reagent (D. melanogaster) | uas-cd8:gfp | PMID:10197526 | ||
| Chemical compound, drug | Philanthotoxin-433 | Santa Cruz Biotechnology | GH28782 | |
| Antibody | Anti-brp (Mouse monoclonal) |
Developmental Studies Hybridoma Bank | RRID:AB_2314866 | IF (1:100) |
| Antibody | Anti-dlg (Rabbit monoclonal) |
PMID:29303480 | IF (1:1000) | |
| Antibody | Cy3 anti-rabbit | Jackson Immuno-research Laboratories | RRID:AB_2338000 | IF (1:500) |
| Antibody | Alexa488 anti-mouse | Jackson Immuno-research Laboratories | RRID:AB_2338840 | IF (1:500) |
| Software, algorithm | Igor Pro 8.03 | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | Graphpad PRISM 7.04 | Graphpad | RRID:SCR_002798 | |
| Software, algorithm | Adobe Illustrator CC 2018 | Adobe | RRID:SCR_010279 | |
| Software, algorithm | MiniAnalysis 6.0.7 | SynaptoSoft | RRID:SCR_002184 | |
| Software, algorithm | SlideBook 6 | Intelligent Imaging | RRID:SCR_014300 | |
| Sequence-based reagent | CREG primer | Applied Biosystems | Dm02135967_g1 | |
| Sequence-based reagent | Ect3 primer | Applied Biosystems | Dm02139373_g1 | |
| Sequence-based reagent | Pepck2 | Applied Biosystems | Dm02366462_s1 | |
| Sequence-based reagent | Cyp6a23 | Applied Biosystems | Dm01824231_g1 | |
| Sequence-based reagent | rpl32 | Applied Biosystems | Dm02151827_g1 | |
| Commercial assay or kit | RNeasy Plus Mini kit | QIAGEN | ID:74134 | |
| Commercial assay or kit | SuperScript III First-Strand synthesis system | Invitrogen | Cat# 18080051 | |
| Commercial assay or kit | TURBO DNA-free kit | ThermoFisher | Cat# AM1907 | |
| Commercial assay or kit | TaqMan Fast Universal PCR Master Mix | Applied Biosystem | Cat# 4352042 | |
| Commercial assay or kit | Lexogen’s Split RNA Extraction Kit | Lexogen | Cat# 008 | |
| Commercial assay or kit | 3’mRNA-Seq Library Prep Kit | Lexogen | Cat# 015 | |
| Commercial assay or kit | Single Cell/Low Input RNA Library Prep Kit | New England Biolabs | Cat# E6420S | |
| Recombinant DNA reagent | pTW (Gateway vector) | DGRC | Cat# 1129 | |
| Recombinant DNA reagent | pENTR-dTOPO Cloning Kit | Invitrogen | Cat# K240020 | |
| Recombinant DNA reagent | Creg cDNA | Drosophila Genomics Resource Center | GH28782 |