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. 2020 Jul 9;9:e54930. doi: 10.7554/eLife.54930

Author response image 1. Enhancing actomyosin activity is not sufficient to generate chiral counterrotating flows during cytokinesis.

Author response image 1.

A, B: Histogram of instantaneous chiral counter-rotating velocity vc for the first, P0 cell division in, control (A) and ect-2 (gof) embryos (B). C, D: Mean myosin concentration profile along the cell division axis of the P0 cell in control and ect-2 (gof) embryos. Vertical black line indicate centre of the cell. E, F: Histogram of instantaneous chiral counter-rotating velocity vc for the first cell division in par-2 (RNAi) embryos in control (E) and ect-2 (gof) embryos (F). Solid lines in A, B, E and F indicate the best fit Gaussian probability density function. Dotted vertical lines indicate mean vc; grey boxes represent the error of the mean at 95% confidence interval. G: Mean myosin concentration profile along the cell division axis of the first cell division in control, par-2 (RNAi) embryos. Vertical black line indicate centre of the cell. Right, normalized myosin concentration levels in the 20% anterior end of the cell compared to 20% of posterior end of the dividing cell. Shaded region indicates error at 95% confidence interval.