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. 2020 Jul 30;11:3796. doi: 10.1038/s41467-020-17606-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Scheme of E. coli chromosome showing two macrodomains, the origin of replication oriC and the site-specific recombination site dif, plus the loci where parS sites have been inserted, called Ori2, Ter3 and Ter4. Positions are indicated in Mbp. b Examples of tracks for Ori2 and Ter4 loci; 2 fps for 20 s, parB-pMT1 system. c Example of 30 MSD from Ter4 (parB-pMT1) locus as a function of lagtime (grey lines), with the median for all MSD of this experiment (n = 1600) shown in blue. d Number of Ter foci per cell (dark blue, cells with no focus; orange, cells with four foci), with ParB-P1 (T3P1) or parB-pMT1 (T4T1). Y axis represents the proportion of cells with that number of foci compared to the total number of cells. e Medians of MSDs as a function of lagtime for Ori2-ParB-P1 (O2P1), Ori2-ParB-pMT1 (O2T1), Ter3-ParB-P1 (T3P1) and Ter4-ParB-pMT1 (T4T1). f Medians of MSD of ParB-P1 and ParB-pMT1 foci at 5 s as a function of intensity of the foci. Error bars show the SEM. For each intensity bin, n = 0, 21, 109, 542, 1512, 3129, 3915, 2876, 1542, 509, 138, 37, 13, 3, 3, 1, 0, 0, 0, 0 foci for O2T1; n = 2, 9, 30, 115, 360, 878, 1399, 1769, 1851, 1635, 1173, 602, 249, 57, 14, 6, 1, 0, 0, 0 for T4T1; n = 0, 4, 26, 125, 363, 665, 830, 638, 372, 152, 36, 13, 4, 1, 0, 1, 0, 0, 0, 0 for O2P1 and n = 0, 3, 20, 84, 231, 395, 532, 514, 438, 317, 216, 88, 71, 26, 10, 2, 1, 0, 0, 0 for T3P1. Source data are provided as a Source Data file.