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. 2020 Jul 30;11:3796. doi: 10.1038/s41467-020-17606-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Scheme of the TPM set-up. A glass coverslip is coated with PEG and neutravidin. A DNA molecule is attached to that surface by a biotin bound to one of its 5′ end. A latex bead coated with anti-digoxigenin is bound to the other extremity of the DNA molecule by the presence of digoxigenin on this 5′ end. The amplitude of the Brownian motion of the bead (A_eq) depends on the size of the DNA molecule that tethers the bead to the glass surface (for details see text and “Methods”). Right: if MatP joins the 2 matS sites together, the A_eq decreases. b Example of a track following a bead tethered to a 2-matS DNA as a function of time. The dotted lines indicate the expected A_eq for a naked DNA (top) or looped DNA (bottom). MatP is added after 2 min (arrow) and the bead tracked for another 13 min. Time scale is 1 min (bar). c Probability distributions of A_eq, before protein injection (light grey histogram), or during the 5 min following the injection (dark grey histograms). The type of DNA (0–2 matS), the type of MatP (wt or Δ20) and the number of tracks obtained for each condition are indicated on the graphs. MatP: *population centred on (mean ± SD, in nm): 248 ± 19; 250 ± 21 and 257 ± 21 for the DNA containing 2, 1 and no matS site, respectively. **population centred on (mean ± SD, in nm): 211 ± 25, 224 ± 26 and 229 ± 28 for the DNA containing 2, 1 and no matS site, respectively. ***population centred on (mean ± SD, in nm): 147 ± 22 and 153 ± 23 for the DNA containing 2 and 1 matS site, respectively. MatPΔ20: *population centred on (mean ± SD): 252 ± 20 nm. Source data are provided as a Source Data file.