Table 3.

Results of the Mediation Model with ESR1 Methylation as Predictor, 95% Bias-Corrected Confidence Interval Predicting Sleep Quality

Outcome Variable: VMS	b	se	t	p	LLCI	ULCI
ESR1-m (ai)	0.0175	0.0078	2.2550	0.0261	0.0021	0.0329
CV
Age	−.0516	0.0169	−3.0573	0.0028	−.0850	−.0182
MS	0.7049	0.1640	4.2969	0.0000	0.3798	1.0300
BMI	0.0194	0.0149	1.2970	0.1974	−.0102	0.0490
E2	−.0018	0.0224	−.0790	0.9372	−.0462	0.0427
Depressive symptoms	−.0151	0.0155	−.9704	0.3340	−.0458	0.0157
Caffeine	0.0201	0.0575	0.3502	0.7269	−.0938	0.1340
Outcome variable: sleep problems (PSQI)
VMS (bi)	0.6301	0.2112	2.9837	0.0035	0.2116	1.0487
CV
Age	0.0586	0.0389	1.5055	0.1351	−.0186	0.1358
MS	−.3619	0.3927	−.9215	0.3588	−1.1401	0.4164
BMI	0.0178	0.0333	0.5331	0.5950	−.0483	0.0838
E2	0.0147	0.0497	0.2962	0.7676	−.0838	0.1132
Depressive symptoms	0.2033	0.0345	5.8904	0.0000	0.1349	0.2717
Caffeine	−.2224	0.1274	−1.7456	0.0837	−.4748	0.0301
Direct/indirect effects on sleep problems (PSQI)
Direct effect (c’)	0.0249	0.0176	1.4159	0.1597	−.0100	0.0597
Indirect effect (aixbi)	0.0110	0.0073			0.0013	0.0299

Notes: a_i represents the association between ESR1-m and VMS; b_i represents the association between VMS and sleep quality; c’ represents the direct effect of ESR1-m on Y, after adjusting for all other variables included in the model; a_ixb_i represents the indirect effect of ESR1-m on sleep quality through VMS; b represents the unstandardized regression coefficient.

Abbreviations: se, standard error, BootLLCI, bootstrapping lower limit confidence interval; BootULCI, bootstrapping upper limit confidence interval; VMS, vasomotor symptoms, ESR1-m, estrogen receptor alpha gene methylation; CV, control variables; MS, menopausal status; BMI, body mass index; E2, estradiol.