Figure 6.
miR-3609 reduced the rate of metabolism and clearance of sorafenib in MHCC97-H cells. MHCC97-H cells were transfected with control, miR-3609, miR-3609 + EPAS-1Mut, or miR-3609 + inhibitor. For the cultured cell model, cells were cultured with 1 μmol/L sorafenib (A and C). For the subcutaneous model, cells were injected into nude mice to form subcutaneous tumors, and the tumor tissues formed by HCC cells were injected with sorafenib solution (B and D). Next, the cell samples and tumor tissues were collected at indicated time points, and the retention of sorafenib in the samples was analyzed by LC-MS/MS. Results are shown as representative images of LC-MS/MS (A and B) and the retention-curves for sorafenib in HCC cultured cells (C) or the subcutaneous tumors (D). *P < 0.05.