Figure 4.

(A) LN cell profiles of WT, γc^KO, and Pim1^Tgγc^KO mice. Contour plots show CD4/CD8 profiles of total LN cells (top) and TCRβ⁺ LN cells (bottom). Gating strategy and percentages of TCRβ⁺ cells are shown in histograms (middle). Data shown are representative of seven independent experiments.

(B) CD4⁺ and CD8⁺ LN T-cell numbers. Data are shown as mean +/− SEM of 11 WT, 6 γc^KO, and 8 Pim1^Tgγc^KO mice, and are pooled from seven experiments performed. *p<0.05, **p<0.01, ***p<0.001, two-tailed Student’s t test.

(C) Ki-67 expression in CD44^lo LN T-cells. Data shown are representative of three independent experiments.

(D) Ki-67 expression in CD44^hi LN T-cells. Data shown are representative of three independent experiments.

(E) BrdU labeling of LN T-cells. LN T-cells were fixed and permeabilized before intra-nuclear staining with anti-BrdU antibodies. Data shown are representative of three mice of each genotype.

(F) Cell size of cultured γc^KO and Pim1^Tgγc^KO CD4⁺ LN T-cells. Freshly isolated LN T-cells were rested in medium for 36 hours. Cell size was assessed by determining FSC values at the end of the culture. Data shown are representative of three independent experiments.