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. 2020 Jul 21;14:32. doi: 10.3389/fnana.2020.00032

Figure 4.

Figure 4

BLA-projection neurons in the CM nucleus are mainly VGluT2-ir glutamatergic neurons. (A–C) After Fluoro-Gold (FG) injection into the BLA, cell bodies co-expressing VGluT2 mRNA signals (A; green) and FG-ir (B; red) in the CM. (C) Merged image of (A,B). Arrows indicating the FG-ir cell bodies co-expressing VGluT2 mRNA signals. Bar = 100 μm in panels (A–C). (D–I) After PHA-L injection into the CM, fluorescence photomicrographs showing axon terminals co-expressing VGluT2 (D; green) and PHA-L (E; red) in the BLA. (F) Merged image of panels (D,E). Bar = 100 μm in panels (D–F). The framed area in panel (F) is magnified in panels (G–I). Arrowheads in panel (I) indicate PHA-L-ir axon terminals co-expressing VGluT2-ir varicosities. Bar = 30 μm in panels (G–I). BLA, basolateral amygdaloid nucleus, anterior part.