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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: Nature. 2020 Jul 22;583(7818):819–824. doi: 10.1038/s41586-020-2504-5

Extended Data Figure 1. Expression patterns of Gad2 and Pvalb across TRN.

Extended Data Figure 1.

(a) Schematic of positions of coronal sections shown along the anterior to posterior axis (indicated by numbers). RNA-FISH co-staining of Gad2 and Pvalb in anterior (b), medial (c), and posterior (d) coronal sections. For each position: Left: overview of the RNA-FISH co-staining in TRN; Right: Zoom-in view of the boxed area in the left panel. For (b), (c), and (d), repeated with n = 2. (e) Representative FACS plot showing gating strategy used in sorting single nuclei for RNA sequencing. Repeated with n = 53 plate-based sorting.