Extended Data Table 1.
Detection of SARS-CoV-2 in the soiled cages.To evaluate transmission potential of SARS-CoV-2 virus via fomites, three naïve fomite contact hamsters were each introduced to a soiled donor cage on 2 dpi. The fomite contact hamsters were single-housed for 48 hours inside the soiled cages and then were each transferred to a new cage on 4 dpi of the donors. The soiled cages were left empty at room temperature and were sampled again on 6 dpi of the donor. Surface samples and corn cob bedding were collected from the soiled cages on different time points to monitor infectious viral load and viral RNA copy numbers in the samples.
| Days post-inoculation | Animal cage info | Sampled area | Material | log10 TCID50/ mL | log10 RNA copies/ mL |
|---|---|---|---|---|---|
| Day 2 | donor cage A | 1.79 | 6.70 | ||
| donor cage B | bedding | corn cobs | < | 5.18 | |
| donor cage C | < | 5.79 | |||
| Day 4 | fomite contact cage A | cage side (in direct contact with the animals) |
< | 6.89 | |
| fomite contact cage B | plastic | < | 5.21 | ||
| fomite contact cage C | 1.79 | 6.33 | |||
| fomite contact cage A | < | 3.76 | |||
| fomite contact cage B | cage lid | plastic | < | 4.33 | |
| fomite contact cage C | < | 4.10 | |||
| fomite contact cage A | < | 5.26 | |||
| fomite contact cage B | pre-filter | paper-based | < | 5.27 | |
| fomite contact cage C | < | 5.31 | |||
| fomite contact cage A | < | 3.64 | |||
| fomite contact cage B | water bottle nozzle | stainless steel | < | 4.20 | |
| fomite contact cage C | 2.21 | 6.06 | |||
| fomite contact cage A | < | 4.84 | |||
| fomite contact cage B | bedding | corn cobs | < | 5.27 | |
| fomite contact cage C | < | 6.06 | |||
| Day 6 | fomite contact cage A | cage side (in direct contact with the animals) |
< | 5.70 | |
| fomite contact cage B | plastic | < | 5.61 | ||
| fomite contact cage C | < | 6.51 | |||
| fomite contact cage A | < | 4.75 | |||
| fomite contact cage B | cage lid | plastic | < | 3.46 | |
| fomite contact cage C | < | 4.24 | |||
| fomite contact cage A | < | 5.48 | |||
| fomite contact cage B | pre-filter | paper-based | < | 5.23 | |
| fomite contact cage C | < | 5.36 | |||
| fomite contact cage A | < | 5.12 | |||
| fomite contact cage B | bedding | corn cobs | < | 6.24 | |
| fomite contact cage C | < | 5.58 |