Table 1.

Top 3 proteins protected by CMS121 from pronase digestion in HT22 and HeLa cells.

HT22	spectral counts		fold change
	control	CMS121	CMS121/control	description
	14	110	7.9	Filamin-B OS = Mus musculus GN = Flnb PE = 1 SV = 3
	48	136	2.8	Filamin, alpha OS = Mus musculus GN = Flna PE = 1 SV = 1
	47	128	2.7	Fatty acid synthase OS = Mus musculus GN = Fasn PE = 1 SV = 2
HeLa	spectral counts		fold change
	control	CMS121	CMS121/control	description
	34	141	4.15	Plectin OS=Homo sapiens GN = PLEC
	41	106	2.59	Fatty acid synthase OS=Homo sapiens GN = Fasn PE = 1 SV = 3
	93	209	2.25	Myosin-9 OS=Homo sapiens GN = MYH9 PE = 1 SV = 4

Mass spectrometry on proteins protected from pronase digestion identified putative target proteins of CMS121. Proteins are considered of interest when they have spectral counts of >100 after CMS121 treatment and at least a 2-fold enrichment in the CMS121-treated samples, compared to vehicle controls, in both cell lines. This threshold was chosen in order to minimize the chances of detecting noise. FASN is the only protein present in the top 3 targets of both cell lines.