Figure 3.

Decoy-CTCFs Suppressed Tumor Proliferation and Migration by Inhibiting SELL

(A and B) qPCR (A) and western blot (B) showed that SELL was highly expressed in ocular melanoma cells. (C and D) qPCR (C) and western blot (D) showed the expression of SELL was significantly decreased in ocular tumor cells after shRNA interference. (E) Plate clone formation assay verified that the proliferation of SELL-interfered group was significantly reduced. (F) Transwell migration assay showed that SELL interference could significantly reduce the migration ability of ocular melanoma. (G) Soft agar assay investigated fewer and smaller colonies in SELL-interfered groups. (H and I) Subcutaneous xenograft model demonstrated that the tumor volume (H) and weight (I) of SELL-interfered group was significantly reduced. (J) The TCGA database of uveal melanoma demonstrated prolonged survival time in patients with low SELL expression.