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Genome editing tools for B. subtilis.
| Genome editing tools | Advantage | Disadvantage | Reference |
|---|---|---|---|
| counter-selectable marker systems | no marker left | limited antibiotic selection markers | [83] |
| site-specific recombination (SSR) systems | eliminate the selection marker | leave remnant sequences | [4] |
| CRISPR/Cas systems | simple and efficient | the off-target effects | [84] |
