Table 4.

Box–Behnken design matrix of ligninase activity optimization.

Coded values					Response
Run	X1	X2	X3	X4	Ligninase activity (μmol/min)
					Observed	Predicted
1	-1	-1	0	0	352.258	341.326
2	-1	+1	0	0	377.419	381.416
3	+1	-1	0	0	366.237	362.635
4	+1	+1	0	0	360.645	372.132
5	0	0	-1	-1	232.043	237.581
6	0	0	-1	+1	341.075	330.071
7	0	0	+1	-1	203.548	215.107
8	0	0	+1	+1	338.280	333.298
9	-1	0	0	-1	218.065	206.907
10	-1	0	0	+1	313.548	318.053
11	+1	0	0	-1	220.860	218.725
12	+1	0	0	+1	304.731	318.260
13	0	-1	-1	0	346.667	364.030
14	0	-1	+1	0	357.850	366.988
15	0	+1	-1	0	408.172	401.405
16	0	+1	+1	0	394.194	379.201
17	-1	0	-1	0	355.699	361.311
18	-1	0	+1	0	346.022	353.838
19	+1	0	-1	0	380.215	369.474
20	+1	0	+1	0	366.237	357.700
21	0	-1	0	-1	223.656	217.234
22	0	-1	0	+1	329.140	323.596
23	0	+1	0	-1	240.430	243.049
24	0	+1	0	+1	343.871	347.368
25	0	0	0	0	360.645	360.828
26	0	0	0	0	353.011	360.828
27	0	0	0	0	368.828	360.828