TABLE 1.

Primers used in this study

Primer	Nucleotide sequence (5′→3′)
Cloning
1-4004-F	GCGCAGCGGCGGCCGCGCTGATACCGCCGCCTTGACATTGATTATTGACTAGTT
1-4004-R	TCAGGAGCTACATTAGGACCATCATCCTCAAGCTCAGAG
4005-7993-F	TGAGGATGATGGTCCTAATGTAGCTCCTGAAACAAATGT
4005-7993-R	ACACACTAACAAAAGCACTATGTAAGCTTGCACCAAAAT
7994-12384-F	CAAGCTTACATAGTGCTTTTGTTAGTGTGTTGTCGAATA
7994-12384-R	GGTTACCTCCTTAACGTGTACCACCTTGCCATCATTGTC
12385-16986-F	GGCAAGGTGGTACACGTTAAGGAGGTAACCGCACAGAAT
12385-16986-R	ACTCAACACGAGCGCGCTGTGGTATGATGCGTGAACATT
16987-21901-F	GCATCATACCACAGCGCGCTCGTGTTGAGTGTTATGATG
16987-21901-R	ATAAGAAATACCATCCTCACCAGCACTAGTAACATTAAG
21902-26980-F	ACTAGTGCTGGTGAGGATGGTATTTCTTATCAACCCTGT
21902-26980-R	ACTGACAGAAGCCATAAAGTTTCTGTTTAGACTAAATGA
26981-28980-F	CTAAACAGAAACTTTATGGCTTCTGTCAGTTTTCAGGAT
26981-28980-R	GCTCACTGACTTTAATTAACTGCGGCGAGGGGCGCGCCACCGCATCCCCAGCAT
Mutagenesis
D350A-F	AAATGGTTTTGCTTTGCCAAGAATCCTACTAATTC
D350A-R	GAATTAGTAGGATTCTTGGCAAAGCAAAACCATTT
G332A-F	GCCATATATGATATTGCCAATCCTAAGGGCATTAG
G332A-R	CTAATGCCCTTAGGATTGGCAATATCATATATGGC
P334A-K335A-F	GATATTGGCAATGCTGCGGGCATTAGATGTGCC
P334A-K335A-R	GGCACATCTAATGCCCGCAGCATTGCCAGCATC
D330A-F	GAAAGCCATATATGCTATTGGCAATCCTAAGGGC
D330A-R	GCCCTTAGGATTGCCAATAGCATATATGGCTTTC
RT-qPCR
IFN-λ1F	GGTGCTGGCGACTGTGATG
IFN-λ1R	GATTGGAACTGGCCCATGTG
IFN-λ3F	ACTTGGCCCAGTTCAAGTCT
IFN-λ3R	CATCCTTGGCCCTCTTGA
IFN-λ4F	GCTATGGGACTGTGGGTCTT
IFN-λ4R	AGGGAGCGGTAGTGAGAGAG
IFN-αF	ACCTTTGCTTTACTGGTGGCC
IFN-αR	ATCTGTGCCAGGAGCATCAAG
IFN-βF	TAGGCGACACTGTTCGTGTTG
IFN-βR	CCAAGCAAGTTGTAGCTCATGG
GAPDH-F	CAATGACCCCTTCATTGACC
GAPDH-R	GACAAGCTTCCCGTTCTCAG
Subgenome-F	AATCCAGGGCCACTTCGAA
Subgenome-R	TTCGCCCTTGGGAATTCTC
Genome-F	TGAAGCCGTCTCATACTATTCTG
Genome-R	AATCCCTCAACAGTGTCAGC