FIG 2.

Inhibition of HIV-1 Env clones by IFITM3. HEK293T cells were cotransfected with NL4-3 ΔNefΔEnv proviral DNA, different HIV-1 Env clones (as indicated), and 100 ng of IFITM3 DNA. The infectivity of viruses was determined by infecting TZM-bl cells. (A) Luciferase data of one representative experiment are shown. (B) Fold inhibition by IFITM3 was determined by calculating the ratio of infectivity of the IFITM3-free virus to those of the IFITM3-bearing virus. Fold inhibition by IFITM3 from three independent transfections are presented. The mean value of fold inhibition for each Env group is indicated by the whisker. The standard deviations are also presented. *, P < 0.05; ns, not significant.