. 2020 Jul 30;94(16):e00514-20. doi: 10.1128/JVI.00514-20

TABLE 1.

Response of T/F Env clones to the inhibition by SERINC5, IFITM3, CD4 MAb (RPA-T4), sCD4, and CCR5 antagonist TAK-779

T/F Env^a	Subtype	Fold inhibition by SERINC5^b	Fold inhibition by IFITM3^c	IC₅₀ values for:^d^,^e
T/F Env^a	Subtype	Fold inhibition by SERINC5^b	Fold inhibition by IFITM3^c	CD4 MAb (μg/ml)	sCD4 (nM)	TAK-779 (μM)
p1012.TC21.32571	B	0.48 ± 0.01	8.09 ± 0.23	0.09	331	0.15
p1006_11.C3.1601	B	0.80 ± 0.20	7.01 ± 0.80	0.05	241	0.06
p1054.TC4.1499	B	1.8 ± 0.53	3.21 ± 0.02	0.11	113	0.05
p1056.TA11.1826	B	0.6 ± 0.42	3.78 ± 0.70	0.06	635	0.4
p1058_11.B11.1550*	B	10.14 ± 1.30	9.22 ± 1.30	0.13	298	0.02
p1059_09.A4.1460	B	3.7 ± 0.09	4.05 ± 0.022	0.13	201	0.09
p6244_13.B5.4576	B	6.0 ± 0.06	5.4 ± 1.04	0.05	254	0.09
p6240_08.TA5.4622	B	0.56 ± 0.01	2.2 ± 0.04	0.06	478	0.14
p63358.p3.4013	B	1.25 ± 0.002	2.6 ± 0.04	0.05	538	0.03
p700010040.C9.4520	B	8.34 ± 0.027	1.53 ± 0.01	0.17	97	0.03
p700010058.A4.4375	B	0.85 ± 0.02	3.20 ± 0.37	0.06	413	0.3
p9014_01.TB1.4769	B	0.99 ± 0.32	4.06 ± 0.35	0.06	>1,000	0.54
p9021_14.B2.4571	B	0.48 ± 0.20	1.82 ± 0.28	0.05	378	0.04
pPRB926_04.A9.4237	B	6.56 ± 0.77	1.13 ± 0.04	0.18	93	0.03
pPRB931_06.TC3.4930	B	0.67 ± 0.15	1.65 ± 0.16	0.12	>1,000	0.16
pPRB958_06.TB1.4305	B	0.81 ± 0.31	3.57 ± 0.02	0.1	141	0.12
pSC05.8C11.2344	B	1.35 ± 0.33	1.14 ± 0.16	ND	ND	ND
pSC45.4B5.2631	B	0.99 ± 0.10	2.96 ± 0.37	0.09	268	0.28
pWEAUd15.410.5017*	B	1.8 ± 0.06	2.40 ± 0.26	ND	ND	ND

All the Env clones are R5 tropic except the two clones marked by *, which are dual tropic.

Fold inhibition by SERINC5 was determined by calculating the ratio of infectivity of the SERINC5-free virus to that of the SERINC5-bearing virus.

Fold inhibition by IFITM3 was determined by calculating the ratio of infectivity of the IFITM3-free virus to that of the IFITM3-bearing virus.

The IC₅₀ values were obtained from the study by Keele et al. (30).

ND, not determined.