FIG 1.

DTMUV infection results in cellular mRNA translation shutoff. DEFs (A) and BHK-21 cells (B) were infected with DTMUV, treated with 1 μg/ml (for DEFs) or 10 μg/ml (for BHK-21 cells) puromycin for 30 min at the indicated time points, harvested, lysed, and analyzed using Western blotting. Puromycin incorporation was detected using a mouse anti-puromycin monoclonal antibody, synthesis of viral proteins was detected using a mouse anti-DTMUV-NS3 polyclonal antibody, and eIF2α phosphorylation was detected using a corresponding rabbit monoclonal antibody. β-Actin was detected as the loading control.