FIG 3.

Cap-independent translation initiation is a strategy shared by different flaviviruses. (A) Schematic presentation of monocistronic reporters for TMUV, JEV, DENV2, and ZIKV. The diagrams are not to scale. (B to D) DEFs (B) and BHK-21 (C) and C6/36 (D) cells were cultured in 48-well plates. When the cells reached ∼70 to 90% confluence, they were transfected with the indicated uncapped reporters. The activities of RLuc measured at 4 h p.t. are presented as means plus SD (n = 3).