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. 2020 Jul 30;94(16):e00609-20. doi: 10.1128/JVI.00609-20

FIG 2.

FIG 2

GW4869 slows HCMV spread. (A) Western blot of intracellular CD63 and CD81 in HCMV-infected (MOI = 1) fibroblasts at 72 hpi treated with DMSO or 8 μM GW4869. Tubulin was used as a loading control. (B) Intracellular and extracellular virus titers from HCMV-infected (MOI = 3) fibroblasts at 96 hpi treated with DMSO or 8 μM GW4869. (C) Trypan blue viability for uninfected or HCMV-infected (MOI = 0.05) fibroblasts at 9 dpi. At indicated time points, cells were trypsinized and resuspended in DMEM before being mixed with trypan blue. Positive-control cells were incubated at 65°C for 10 min. (D) Virus spread in HCMV-infected (MOI = 0.05) fibroblasts treated with DMSO or 8 μM GW4869. Relative infection measured by GFP expression in the monolayer. Values for titers and virus spread are from three independent experiments. (E) Fluorescence images from low-MOI spread infection in panel D. Scale bars are 1,000 μm. (F) Total cell count of uninfected and HCMV-infected (MOI = 0.05) fibroblasts at 9 dpi treated with DMSO or 8 μM GW4869. *, P < 0.05.