EVs from HCMV-infected cells contain viral proteins. (A) Table of late domain sequences within viral proteins detected in infected EVs. (B) Western blot analysis of viral and cellular proteins in pooled EV fractions from uninfected or HCMV-infected (MOI = 1) fibroblasts at 72 hpi. Samples loaded at equal volumes. (C) Amino acid sequences of HCMV strains AD169 (GenBank FJ527563.1), Merlin (NCBI Reference Sequence NC_006273.2), TB40/E (GenBank EF999921.1), Toledo (GenBank GU937742.2), Towne (GenBank FJ616285.1), and TR (GenBank KF021605.1). Amino acid positions are shown in parentheses. Potential late domains are shown in red. *, stop codon.