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. 2020 Jul 30;94(16):e00609-20. doi: 10.1128/JVI.00609-20

FIG 6.

FIG 6

Knockdown of CD63 does not inhibit HCMV spread. (A) Western blot analysis of CD63 in uninfected fibroblasts transduced with lentivirus expressing shRNA to CD63 or scrambled control. Tubulin was used as a loading control. (B) Intracellular and extracellular virus titers from HCMV-infected (MOI = 3) transduced fibroblasts at 96 hpi. Titers are from three independent experiments. (C) Virus spread in HCMV-infected (MOI = 0.05) transduced fibroblasts. Relative infection measured by GFP expression in the monolayer. Values for low-MOI spread data represent five independent experiments. (D) Western blot analysis of CD63 in uninfected fibroblasts transduced with lentivirus expressing shRNA clones to CD63 or scrambled control. (E) Intracellular and extracellular virus titers from HCMV-infected (MOI = 3) transduced fibroblasts at 96 hpi. Titers are from three independent experiments.