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. 2020 Jul 30;94(16):e00609-20. doi: 10.1128/JVI.00609-20

FIG 7.

FIG 7

EVs from HCMV-infected cells enhance virus spread. (A) Experimental outline to transfer purified EVs to HCMV-infected (MOI = 0.05) fibroblasts treated with 8 μM GW4869; 3,000 to 5,000 purified EVs were added to spread experiments at 3, 4, and 5 dpi as indicated, and GFP expression was measured at 3, 6, 9, 12, and 15 dpi. (B) Fold increases for four individual experiments showing virus spread relative to that for GW4869-treated HCMV-infected fibroblasts. (C) Fluorescence images from the spread infection in panel B. Scale bar is 250 μm. (D) Virus spread in fibroblasts treated with 8 μM GW4869 and EVs purified from cells treated with 8 μM GW4869. *, P < 0.05 compared to untreated control. (E) Virus spread in the presence of EVs purified from HCMV infection. *, P < 0.05. (F) Virus spread in the presence of EVs purified from uninfected fibroblasts.