FIG 5.

IRES-directed translation initiation efficiency. Monolayers of BHK-21 or IBRS-2 cells were transfected with the dual-luciferase reporter pCMV-RHF-IRES(WT) or pCMV-RHF-IRES(C351G), respectively. (A) IRES-directed translation initiation efficiency was assessed by measuring the yield ratio of FLuc expression to RLuc expression at 24 h posttransfection. BHK-21 or IBRS-2 cells were infected with rC351G or FMDV(WT) at 33°C, 37°C, or 41°C, and the resulting virus was harvested at 24 h and analyzed for VP2 protein expression by Western blotting (B) and for virus titer detection by TCID₅₀ assay (C). The mean values ± standard deviations are shown (n = 3). ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (determined by two-way analysis of variance [ANOVA] test followed by Bonferroni’s post hoc analysis).