TABLE 2.
Virus derived from infectious cDNA
| Polymerasea | Ribavirin selected | Engineered variant | Stably maintained | Reference |
|---|---|---|---|---|
| WT | Yes | 74 | ||
| G64S | ✓ | Yes | 54 | |
| L420A | ✓ | Yes | 19 | |
| G64S L420A | ✓ | Yes | 18 | |
| L420V | ✓ | Yes | This study | |
| L420I | ✓ | Yes | This study | |
| M392A | ✓ | Yes | This study | |
| M392L | ✓ | Yes | This study | |
| M392V | ✓ | Yes | This study | |
| K375R | ✓ | Yes | This study | |
| R376K | ✓ | Yes | This study | |
| K375A | ✓ | No | This study | |
| R376A | ✓ | No | This study | |
| M299I | ✓ | Yes | This study | |
| M323I | ✓ | Yes | This study | |
| T353I | ✓ | Yes | This study | |
| Y275H | ✓ | Yes | 56 |
a
Polymerase mutations identified during ribavirin selection, along with engineered variants, were cloned into infectious cDNA. Poliovirus was recovered from HeLa cells transfected with RNA derived from each clone. cDNA from the 3Dpol region was sequenced to determine whether mutations were stably maintained in virus.