TABLE 3.
Biochemical phenotypes of purified polymerases
| Polymerasea | Initiation (min) | ECb stability (min) | Processive rate (nt/s) | Processive Km (μM) | Single NTP rate (per s) | Single NTP Km (μM) | Discrimination factor |
|---|---|---|---|---|---|---|---|
| From Kempf et al.c | |||||||
| WT | 6 ± 1 | 88 ± 9 | 22 ± 1 | 67 ± 2 | 30 ± 1 | 37 ± 2 | 120 ± 10 |
| G64S | 8 ± 1 | 24 ± 7 | 12 ± 1 | 46 ± 4 | 23 ± 1 | 39 ± 5 | 220 ± 40 |
| L420A | 8 ± 1 | 28 ± 4 | 30 ± 1 | 75 ± 3 | 40 ± 1 | 39 ± 3 | 100 ± 10 |
| G64S L420A | 6 ± 3 | 40 ± 8 | 26 ± 1 | 84 ± 9 | 28 ± 1 | 36 ± 6 | 220 ± 50 |
| This study | |||||||
| WT | 4 ± 1 | 130 ± 20 | 26 ± 1 | 72 ± 4 | 40 ± 1 | 46 ± 3 | 100 ± 10 |
| L420V | 4 ± 1 | 170 ± 10 | 29 ± 1 | 82 ± 5 | 41 ± 1 | 40 ± 3 | 99 ± 9 |
| L420I | 4 ± 1 | 60 ± 2 | 33 ± 1 | 90 ± 5 | 45 ± 1 | 41 ± 2 | 107 ± 8 |
| M392A | 3 ± 1 | 20 ± 1 | 30 ± 1 | 90 ± 8 | 33 ± 1 | 38 ± 3 | 120 ± 10 |
| M392L | 4 ± 1 | 39 ± 4 | 25 ± 1 | 74 ± 9 | 38 ± 1 | 34 ± 1 | 102 ± 6 |
| M392V | 4 ± 1 | 11 ± 1 | 22 ± 1 | 74 ± 7 | 42 ± 1 | 51 ± 3 | 160 ± 10 |
| K375R | 5 ± 1 | 51 ± 2 | 20 ± 1 | 96 ± 8 | 36 ± 1 | 44 ± 3 | 200 ± 20 |
| R376K | 2 ± 1 | 30 ± 3 | 65 ± 2 | 160 ± 10 | 48 ± 1 | 46 ± 3 | 17 ± 1 |
| M299I | 4 ± 1 | 180 ± 30 | 18 ± 1 | 66 ± 4 | 35 ± 1 | 42 ± 2 | 120 ± 10 |
| M323I | 4 ± 1 | 150 ± 20 | 18 ± 1 | 64 ± 4 | 37 ± 1 | 50 ± 3 | 130 ± 10 |
| T353I | 5 ± 1 | 90 ± 20 | 18 ± 1 | 73 ± 2 | 38 ± 1 | 49 ± 3 | 160 ± 10 |
| Y275H | 13 ± 6 | 95 ± 3 | 27 ± 1 | 95 ± 8 | 35 ± 1 | 42 ± 2 | 140 ± 10 |