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. 2020 Jul 12;41:101052. doi: 10.1016/j.molmet.2020.101052

Figure 2.

Figure 2

Over-expression of 14-3-3ζ improves tolerance to prolonged cold exposure in male mice. (A) Wildtype (WT) and 14-3-3ζ over-expressing (TAP) mice were implanted with a temperature probe 1 week before placement in CLAMS metabolic cages for 3 days at 4 °C. (B,C) Body weights (B) and lean and fat mass measurements by echo MRI (C) were obtained before and after the prolonged cold challenge. (D–F) During the light and dark cycles at 22 °C and 4 °C, food intake (G), locomotor activity (H), and RER were measured in WT and TAP mice. (G–J) Body temperature (G,H) and energy expenditure, relative to the lean mass of mice (I,J), were measured and are reported either as the average trace for all mice over the cold challenge (G, I) or as an average per light:dark cycle (H,J; n = 8 WT and n = 10 TAP mice; ∗: p < 0.05 when compared to WT). Data are represented as mean ± SEM.