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. 2020 Jul 12;41:101052. doi: 10.1016/j.molmet.2020.101052

Figure 4.

Figure 4

Transgenic over-expression of 14-3-3ζ increases beiging of ingulnal white adipocytes in male mice. (A,B) After 3 days of cold exposure, BAT (A) and inguinal white adipose tissue (iWAT, B) from wildtype (WT) and transgenic mice over-expressing 14-3-3ζ (TAP) were harvested, followed by quantitative PCR to measure genes associated with thermogenesis and beiging (n = 8 WT and 10 TAP; ∗: p < 0.05). (C) Immunohistochemistry was used to detect UCP1 immunoreactivity in paraffin embedded iWAT sections from WT or TAP mice exposed to prolonged cold (representative images for n = 8 WT and 10 TAP mice; scale bar = 50 μm). (D) Immunoblotting was used to detect UCP1 protein in lysates from the iWAT, gWAT, or BAT of prolonged cold-exposed WT and TAP mice (representative image of n = 6 mice per genotype). (E)Fgf21 mRNA levels were measured from iWAT harvested from WT and TAP mice exposed for 3 days to (n = 6 per group, ∗: p < 0.05). Data are represented as mean ± SEM.