Fig. 3.

A) Detection of LPS-induced secretion of pro-inflammatory cytokines in EGV-4 T cells. Cells were stimulated with LPS at indicated concentrations for 24 h. The protein levels of TNFα, IL6, and IL33 in cell culture supernatants and cell extracts were analyzed with western blotting. (B) The density of bands was quantified by densitometry, and was corrected with the density of GAPDH as loading control. Relative levels of TNFα, IL6, and IL33 are shown as a ratio to respected levels of untreated cells. Each value represents the mean ± SD (n = 7).