Table 2.
Analysis of variance (ANOVA) for the response surface quadratic model
| Source | Sum of squares | df | Mean square | F value | p value | |
|---|---|---|---|---|---|---|
| Model | 4906.49 | 14 | 350.46 | 397.82 | < 0.0000 | significant |
| A-Enzyme conc | 520.08 | 1 | 520.08 | 590.36 | < 0.0001 | |
| B-MNPs conc | 574.08 | 1 | 574.08 | 651.66 | < 0.0001 | |
| C-Cross linking (carbodiamide) | 720.75 | 1 | 720.75 | 818.15 | < 0.0001 | |
| D-Time course of cross linking | 80.08 | 1 | 80.08 | 90.91 | < 0.0001 | |
| AB | 0.2500 | 1 | 0.2500 | 0.2838 | 0.6026 | |
| AC | 6.25 | 1 | 6.25 | 7.09 | 0.0185 | |
| AD | 6.25 | 1 | 6.25 | 7.09 | 0.0185 | |
| BC | 30.25 | 1 | 30.25 | 34.34 | < 0.0001 | |
| BD | 0.2500 | 1 | 0.2500 | 0.2838 | 0.6026 | |
| CD | 56.25 | 1 | 56.25 | 63.85 | < 0.0001 | |
| A2 | 1920.82 | 1 | 1920.82 | 2180.39 | < 0.0001 | |
| B2 | 966.77 | 1 | 966.77 | 1097.41 | < 0.0001 | |
| C2 | 1006.77 | 1 | 1006.77 | 1142.82 | < 0.0001 | |
| D2 | 410.82 | 1 | 410.82 | 466.34 | < 0.0000 | significant |
| Residual | 12.33 | 14 | 0.8810 | – | – | |
| Lack of fit | 12.33 | 10 | 1.23 | – | – | |
| Pure error | 0.0000 | 4 | 0.0000 | – | – | |
| Cor total | 4918.83 | 28 |