Table 3.

Summary of resazurin reduction assays for chordoma cell lines.

Drug	U-CH1				U-CH2
	IC50 (µM, absolute)	IC50 (µM, relative)	Maximal inhibition (%)	n	IC50 (µM, absolute)	IC50 (µM, relative)	Maximal inhibition (%)	n
Afatinib	0.54	0.38	93	6	9.4	8.8	98	7
Palbociclib	11.4	10.8	96	6	20.8	19.5	94	6
RDEA119	–	2.4	59	2	24.5	24.4	94	4
AZD0530	2.7	1.5	85	5	29.4	26.6	100	3
AZD2014	0.94	0.35	72	7	–	0.61	53	8
AZD4054	11.9	11.0	84	2	18.8	19.2	100	2

Data generated includes geometric mean IC₅₀, and arithmetic mean of maximal inhibition. Absolute IC₅₀ is the dose that reduces resazurin fluorescence by 50%, relative to positive and negative controls. Relative IC₅₀ is the dose that has 50% of the maximal inhibition for a given drug. Where values are absent this represents the drug never reaching 50% inhibition in several replicate experiments.