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. 2020 Jul 31;3:414. doi: 10.1038/s42003-020-01151-5

Fig. 4. No significant difference in fiber orientation and alignment were found between synthesized SHG images and real SHG images of an independent pancreatic TMA slide.

Fig. 4

Top row shows the ROI selection for fiber metrics analysis based on 30% SHG signal for synthesized SHG (a) and real SHG (b). Bottom row shows the Bland-Altman analysis plot for fiber alignment inside ROI as the difference of two measurement vs. the mean (c) and the same plot for sin() of orientation of the fibers inside blocks (d). Each block is 256 × 256 pixels (110 × 110 µm).