Table 2.
Intraday and Interday precisions associated with this method.
| Fatty acid standard | Concentration | Diagnostic fragments (m/z) | Intraday RSD (%) without I.S. (%) | Intraday RSD (%) with I.S.a (%) | Interday RSD (%) without I.S. (%) | Interday RSD (%) with I.S.a (%) |
|---|---|---|---|---|---|---|
| cis-Vaccenic acid | 10 μM | 183.14 | 5.9 | 6.2 | 26.5 | 8.3 |
| C18:1 cis-9 | 199.13 | 6.9 | 5.4 | 26.4 | 4.7 | |
| 100 μM | 183.14 | 4.2 | 7.3 | 28.0 | 9.2 | |
| 199.13 | 3.6 | 8.0 | 28.4 | 5.6 | ||
| γ-Linolenic acid | 10 μM | 129.09 | 1.3 | 4.0 | 35.7 | 13.7 |
| C18:3 cis-6, 9, 12 | 141.09 | 8.3 | 9.8 | 31.2 | 6.1 | |
| 181.12 | 11.8 | 12.7 | 32.4 | 6.7 | ||
| 100 μM | 129.09 | 5.7 | 10.9 | 31.6 | 15.0 | |
| 141.09 | 3.9 | 9.0 | 30.3 | 12.4 | ||
| 181.12 | 4.7 | 10.4 | 30.1 | 12.6 |
aFragment ions of the internal standard, 13C-labeled oleic acid, was used for normalization.