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. 2020 Jul 31;10:12912. doi: 10.1038/s41598-020-69396-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of mouse skin proteinases. (A) Gelatin zymography of mouse skin proteins. Protein samples (30 μg) from control skin proteins (C) or hemorrhagic skin proteins (H), prepared under non-reducing conditions, were submitted to electrophoresis on 10% SDS–polyacrylamide copolymerized with gelatin. Gels were stained with Coomassie blue. Proteins with activity were identified as clear zones of lysis against a dark background. White arrows indicate bands of gelatinolytic activity. (B) Detection of the presence of MMP9 in the mouse skin, as shown by Western blot analysis. Protein samples (30 μg) from control skin proteins (C) or hemorrhagic skin proteins (H) were immunostained with anti-MMP9 antibodies. Numbers on the left indicate molecular mass marker mobility.