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. 2020 Jul 31;10:12922. doi: 10.1038/s41598-020-69472-3

Figure 3.

Figure 3

MS diet decreases SIgA secretion and E. coli load in mice stools. (a) ELISA quantification of SIgA in fecal pellet of mice fed a control or MS diet (n = 6). (b) SIgA-coated bacteria were quantified in the stools of mice using flow cytometry. Anti-SIgA-PE antibody was used to detect IgA + bacteria. The data represent the percentage of IgA + bacteria in control diet (CTR) and MS diet groups, over the total number of events detected (n = 10 for each group). (c) qPCR relative quantification of E. coli in stools, standardized to the total copy number of 16S in mice fed CTR (n = 16) and MS diet (n = 16). (d) Quantification of Gram negative bacilli count on Drigalsky agar plate in stools from mice fed CTR (n = 16) and MS diet (n = 16). (e) Immunofluorescence staining of E. coli associated to colonic mucosa. E. coli bacteria were stained in green, DNA was stained in blue. White arrows indicate E. coli bacteria. Mann–Whitney test. ns non-significant. **p < 0.01; ****p < 0.0001.